"The recent escalation of violence in eastern Ukraine, and its impact on the civilian population, is of great concern," said Commissioner Christos Stylianides.
"Here today in Bakhmut, I want to tell the Ukrainian people: you are not alone," he said. "All civilians in need must be helped, on both sides of the contact line."
He said that the new aid package would "help humanitarian partners in eastern Ukraine to cover urgent medical, shelter, water and sanitation needs."
Read alsoUN Resident Coordinator warns of critical humanitarian situation in Donbas"It is crucial that aid gets to all vulnerable people affected by the conflict swiftly, safely and impartially," Stylianides said.
Around 50% of the Commission's humanitarian assistance targets people in need in non-government controlled areas, the press service reported.
Together with the funding provided directly by the Member States, the EU as a whole has directed over EUR 399 million in humanitarian and recovery aid to those affected by the conflict since early 2014, according to the report.
Read alsoPoroshenko: Putin wants to have Ukrainian Aleppo in Donbas – mediaAn estimated 2.8 million people are displaced and in need of humanitarian assistance in Ukraine and neighbouring countries.